Name: FEZF1 Antibody (PACO60701)
SKU: PACO60701
Availability: OutOfStock

Antibody Name:	FEZF1 Antibody (PACO60701)
Antibody SKU:	PACO60701
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Fez family zinc finger protein 1 protein (1-115AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: U251 whole cell lysate. All lanes: FEZF1 antibody at 6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 53, 47, 52 kDa. Observed band size: 53 kDa.

IHC image of PACO60701 diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Transcription repressor. Involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation. Expression is required in OSN to cell-autonomously regulate OSN axon projections. Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. May be required for correct rostral migration of the interneuron progenitors (By similarity).

Synonyms:

Fez family zinc finger protein 1 (Zinc finger protein 312B), FEZF1, FEZ ZNF312B

UniProt Protein Function:	FEZF1: Transcription repressor. Involved in the axonal projection and proper termination of olfactory sensory neurons (OSN). Plays a role in rostro-caudal patterning of the diencephalon and in prethalamic formation. Expression is required in OSN to cell-autonomously regulate OSN axon projections. Regulates non-cell-autonomously the layer formation of the olfactory bulb development and the interneurons. May be required for correct rostral migration of the interneuron progenitors. Belongs to the krueppel C2H2-type zinc-finger protein family. 3 isoforms of the human protein are produced by alternative splicing.Protein type: C2H2-type zinc finger protein; Cell development/differentiation; Transcription factor; Motility/polarity/chemotaxisChromosomal Location of Human Ortholog: 7q31.32Biological Process: axon guidance; negative regulation of transcription, DNA-dependentDisease: Hypogonadotropic Hypogonadism 22 With Or Without Anosmia
UniProt Protein Details:
NCBI Summary:	This gene encodes a transcriptional repressor that belongs to the zinc finger double domain protein family. The encoded protein is thought to play a role in the embryonic migration of gonadotropin-releasing hormone neurons into the brain. Mutations in this gene are associated with hypogonadotropic hypogonadism-22 with anosmia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
UniProt Code:	A0PJY2
NCBI GenInfo Identifier:	152032459
NCBI Gene ID:	389549
NCBI Accession:	A0PJY2.1
UniProt Secondary Accession:	A0PJY2,A0PJY3, A4D0W3, B4DUP9, B7ZM98
UniProt Related Accession:	A0PJY2
Molecular Weight:	51,651 Da
NCBI Full Name:	Fez family zinc finger protein 1
NCBI Synonym Full Names:	FEZ family zinc finger 1
NCBI Official Symbol:	FEZF1
NCBI Official Synonym Symbols:	FEZ; HH22; ZNF312B
NCBI Protein Information:	fez family zinc finger protein 1
UniProt Protein Name:	Fez family zinc finger protein 1
UniProt Synonym Protein Names:	Zinc finger protein 312B
Protein Family:	Fez family zinc finger protein
UniProt Gene Name:	FEZF1
UniProt Entry Name:	FEZF1_HUMAN