Name: FAU Antibody (PACO60697)
SKU: PACO60697
Availability: OutOfStock

Antibody Name:	FAU Antibody (PACO60697)
Antibody SKU:	PACO60697
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human 40S ribosomal protein S30 protein (1-59AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO60697 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of A549 cells with PACO60697 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	cytosol, cytosolic small ribosomal subunit, extracellular space, nucleoplasm, small ribosomal subunit, RNA binding, structural constituent of ribosome, antibacterial humoral response, antimicrobial humoral immune response mediated by antimicrobial peptide, defense response to Gram-positive bacterium
Synonyms:	40S ribosomal protein S30 (Small ribosomal subunit protein eS30), FAU

UniProt Protein Function:	RPS30: This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin. [provided by RefSeq, Jul 2008]Protein type: Ribosomal; Translation; ApoptosisChromosomal Location of Human Ortholog: 11q13
UniProt Protein Details:
NCBI Summary:	This gene is the cellular homolog of the fox sequence in the Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV). It encodes a fusion protein consisting of the ubiquitin-like protein fubi at the N terminus and ribosomal protein S30 at the C terminus. It has been proposed that the fusion protein is post-translationally processed to generate free fubi and free ribosomal protein S30. Fubi is a member of the ubiquitin family, and ribosomal protein S30 belongs to the S30E family of ribosomal proteins. Whereas the function of fubi is currently unknown, ribosomal protein S30 is a component of the 40S subunit of the cytoplasmic ribosome and displays antimicrobial activity. Pseudogenes derived from this gene are present in the genome. Similar to ribosomal protein S30, ribosomal proteins S27a and L40 are synthesized as fusion proteins with ubiquitin. [provided by RefSeq, Nov 2014]
UniProt Code:	P62861
NCBI GenInfo Identifier:	51338655
NCBI Gene ID:	2197
NCBI Accession:	P62861.1
UniProt Secondary Accession:	P62861,Q05472, Q95261, Q9H5V4
UniProt Related Accession:	P62861,P35544, P62861
Molecular Weight:	6,648 Da
NCBI Full Name:	40S ribosomal protein S30
NCBI Synonym Full Names:	FAU, ubiquitin like and ribosomal protein S30 fusion
NCBI Official Symbol:	FAU
NCBI Official Synonym Symbols:	S30; FAU1; Fub1; Fubi; asr1; RPS30; MNSFbeta
NCBI Protein Information:	ubiquitin-like protein fubi and ribosomal protein S30
UniProt Protein Name:	40S ribosomal protein S30
UniProt Synonym Protein Names:
Protein Family:	40S ribosomal protein
UniProt Gene Name:	FAU
UniProt Entry Name:	RS30_HUMAN