Name: FAN1 Antibody (PACO58997)
SKU: PACO58997
Availability: OutOfStock

Antibody Name:	FAN1 Antibody (PACO58997)
Antibody SKU:	PACO58997
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Fanconi-associated nuclease 1 protein (167-373AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: PC-3 whole cell lysate. All lanes: FAN1 antibody at 5.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 115, 60 kDa. Observed band size: 115 kDa.

IHC image of PACO58997 diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL. Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap. Also has endonuclease activity toward 5'-flaps.

Synonyms:

Fanconi-associated nuclease 1 (EC 3.1.21) (EC 3.1.4.1) (FANCD2/FANCI-associated nuclease 1) (hFAN1) (Myotubularin-related protein 15), FAN1, KIAA1018 MTMR15

UniProt Protein Function:	FAN1: Nuclease required for maintenance of chromosomal stability. Plays a key role in DNA repair of DNA interstrand cross-links (ICL) by being recruited to sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL- induced DNA breaks by being required for efficient homologous recombination, possibly in the resolution of homologous recombination intermediates. Not involved in DNA double-strand breaks resection. Has both endonuclease activity toward 5'-flaps and 5'-exonuclease activity: may act in concert with the 3'-flap- specific enzymes to unhook the ICL by cleaving the lagging-strand template. Belongs to the FAN1 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 3.1.4.1; Deoxyribonuclease; Ubiquitin conjugating system; DNA repair, damageChromosomal Location of Human Ortholog: 15q13.2-q13.3Cellular Component: cytoplasm; intercellular bridge; nucleoplasm; nucleusMolecular Function: 5'-3' exonuclease activity; 5'-flap endonuclease activity; magnesium ion binding; protein binding; ubiquitin bindingBiological Process: DNA repair; double-strand break repair via homologous recombination; nucleotide-excision repair; nucleotide-excision repair, DNA incisionDisease: Interstitial Nephritis, Karyomegalic
UniProt Protein Details:
NCBI Summary:	This gene plays a role in DNA interstrand cross-link repair and encodes a protein with 5' flap endonuclease and 5'-3' exonuclease activity. Mutations in this gene cause karyomegalic interstitial nephritis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Feb 2016]
UniProt Code:	Q9Y2M0
NCBI GenInfo Identifier:	160410012
NCBI Gene ID:	22909
NCBI Accession:	Q9Y2M0.4
UniProt Secondary Accession:	Q9Y2M0,Q86WU8, A8K4M2
UniProt Related Accession:	Q9Y2M0
Molecular Weight:	59,498 Da
NCBI Full Name:	Fanconi-associated nuclease 1
NCBI Synonym Full Names:	FANCD2/FANCI-associated nuclease 1
NCBI Official Symbol:	FAN1
NCBI Official Synonym Symbols:	KMIN; hFAN1; MTMR15; KIAA1018
NCBI Protein Information:	fanconi-associated nuclease 1
UniProt Protein Name:	Fanconi-associated nuclease 1
UniProt Synonym Protein Names:	FANCD2/FANCI-associated nuclease 1
Protein Family:	Fanconi-associated nuclease
UniProt Gene Name:	FAN1
UniProt Entry Name:	FAN1_HUMAN