FAM89B Antibody (PACO39926)
- SKU:
- PACO39926
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | FAM89B Antibody (PACO39926) |
| Antibody SKU: | PACO39926 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:20-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Leucine repeat adapter protein 25 protein (1-162AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO39926 diluted at 1:50 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of A549 cells with PACO39926 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Negatively regulates TGF-beta-induced signaling; in cooperation with SKI prevents the translocation of SMAD2 from the nucleus to the cytoplasm in response to TGF-beta. |
| Synonyms: | Leucine repeat adapter protein 25, FAM89B, Lrap25 |
| UniProt Protein Function: | FAM89B: Belongs to the FAM89 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Receptor, misc.Chromosomal Location of Human Ortholog: 11q13Cellular Component: cell surface; cytoplasmBiological Process: negative regulation of transforming growth factor beta receptor signaling pathway |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q8N5H3 |
| NCBI GenInfo Identifier: | 149192858 |
| NCBI Gene ID: | 23625 |
| NCBI Accession: | NP_001092255.1 |
| UniProt Secondary Accession: | Q8N5H3,Q6PJ27, E9PB01, E9PL72 |
| UniProt Related Accession: | Q8N5H3 |
| Molecular Weight: | 15,391 Da |
| NCBI Full Name: | protein FAM89B isoform 1 |
| NCBI Synonym Full Names: | family with sequence similarity 89, member B |
| NCBI Official Symbol: | FAM89B |
| NCBI Official Synonym Symbols: | MTVR; MTVR1; LRAP25 |
| NCBI Protein Information: | protein FAM89B |
| UniProt Protein Name: | Protein FAM89B |
| UniProt Synonym Protein Names: | |
| Protein Family: | |
| UniProt Gene Name: | FAM89B |
| UniProt Entry Name: | FA89B_HUMAN |
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