FAM83D Antibody (PACO60412)
- SKU:
- PACO60412
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | FAM83D Antibody (PACO60412) |
| Antibody SKU: | PACO60412 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein FAM83D protein (339-470AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: K562 whole cell lysate, 293T whole cell lysate. All lanes: FAM83D antibody at 4.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 65, 63 kDa. Observed band size: 65 kDa. |
 | Immunofluorescence staining of HepG2 cells with PACO60412 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Probable proto-oncogene that regulates cell proliferation, growth, migration and epithelial to mesenchymal transition. Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC. May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade. May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22. |
| Synonyms: | Protein FAM83D (Spindle protein CHICA), FAM83D, C20orf129 |
| UniProt Protein Function: | FAM83D: Required for proper chromosome congression and alignment during mitosis. Required for targeting KIF22/KID to the spindle microtubules. Belongs to the FAM83 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 20q11.23Cellular Component: cytoplasmMolecular Function: kinesin binding; microtubule binding; protein binding; protein kinase bindingBiological Process: cell migration; cell proliferation; epithelial to mesenchymal transition; metaphase plate congression; regulation of protein catabolic process; regulation of TOR signaling pathway |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9H4H8 |
| NCBI GenInfo Identifier: | 25452904 |
| NCBI Gene ID: | 81610 |
| NCBI Accession: | Q9H4H8.3 |
| UniProt Secondary Accession: | Q9H4H8,Q5THR2, Q68EN1, Q6P457, Q7Z6H0, Q96DF5, Q96N89 Q9BVM8, B4E1I7 |
| UniProt Related Accession: | Q9H4H8 |
| Molecular Weight: | 62,596 Da |
| NCBI Full Name: | Protein FAM83D |
| NCBI Synonym Full Names: | family with sequence similarity 83 member D |
| NCBI Official Symbol: | FAM83D |
| NCBI Official Synonym Symbols: | CHICA; C20orf129; dJ616B8.3 |
| NCBI Protein Information: | protein FAM83D |
| UniProt Protein Name: | Protein FAM83D |
| UniProt Synonym Protein Names: | Spindle protein CHICA |
| Protein Family: | Protein |
| UniProt Gene Name: | FAM83D |
| UniProt Entry Name: | FA83D_HUMAN |
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