FAM50B Antibody (PACO62655)
- SKU:
- PACO62655
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | FAM50B Antibody (PACO62655) |
| Antibody SKU: | PACO62655 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein FAM50B protein (98-199AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO62655 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of Hela cells with PACO62655 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | intercellular bridge, nucleoplasm |
| Synonyms: | Protein FAM50B (Protein XAP-5-like), FAM50B, X5L |
| UniProt Protein Function: | FAM50B: Belongs to the FAM50 family.Protein type: Unknown functionChromosomal Location of Human Ortholog: 6p25.2Cellular Component: nucleus |
| UniProt Protein Details: | |
| NCBI Summary: | |
| UniProt Code: | Q9Y247 |
| NCBI GenInfo Identifier: | 6912326 |
| NCBI Gene ID: | 26240 |
| NCBI Accession: | NP_036267.1 |
| UniProt Secondary Accession: | Q9Y247,Q5T2L6 |
| UniProt Related Accession: | Q9Y247 |
| Molecular Weight: | 38,709 Da |
| NCBI Full Name: | protein FAM50B |
| NCBI Synonym Full Names: | family with sequence similarity 50, member B |
| NCBI Official Symbol: | FAM50B |
| NCBI Official Synonym Symbols: | X5L; D6S2654E |
| NCBI Protein Information: | protein FAM50B |
| UniProt Protein Name: | Protein FAM50B |
| UniProt Synonym Protein Names: | Protein XAP-5-like |
| Protein Family: | Protein |
| UniProt Gene Name: | FAM50B |
| UniProt Entry Name: | FA50B_HUMAN |
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