Antibody Name:	FAM20C Antibody (PACO58280)
Antibody SKU:	PACO58280
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000
Species Reactivity:	Human
Immunogen:	Recombinant Human Extracellular serine/threonine protein kinase FAM20C protein (107-224AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: HepG2 whole cell lysate. All lanes: FAM20C antibody at 4.1µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 67, 30 kDa. Observed band size: 67 kDa.
	IHC image of PACO58280 diluted at 1:500 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO58280 diluted at 1:500 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth. Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome. Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity. Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1. In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion.

Synonyms:

Extracellular serine/threonine protein kinase FAM20C (EC 2.7.11.1) (Dentin matrix protein 4) (DMP-4) (Golgi casein kinase) (Golgi-enriched fraction casein kinase) (GEF-CK), FAM20C, DMP4

UniProt Protein Function:	FAM20C: an atypical serine protein kinase that localizes within the Golgi apparatus and is secreted. Distantly related to the Drosophila Golgi-localized kinase Four-jointed. Phosphorylates secretory proteins including the caseins and members of the small integrin-binding ligand, N-linked glycoprotein (SIBLING) protein family, which modulate biomineralization. Colocalizes with the Golgi resident protein GM130. Preferentially phosphorylates its targets within the S-x-E/pS motif. Requires Mn2+ as an alternative to Mg2+ as the activating cation. Defects in FAM20C are the cause of Raine syndrome (RNS). RNS is an autosomal recessive osteosclerotic bone dysplasia with neonatal lethal outcome. Clinical features include generalized osteosclerosis, craniofacial dysplasia and microcephaly. Two isoforms of the human protein are produced by alternative splicing.Protein type: EC 2.7.11.1; Secreted, signal peptide; Secreted; Protein kinase, Ser/Thr (non-receptor)Chromosomal Location of Human Ortholog: 7p22.3Cellular Component: extracellular space; Golgi apparatusMolecular Function: manganese ion binding; protein binding; protein serine/threonine kinase activityBiological Process: biomineral formation; protein amino acid phosphorylationDisease: Raine Syndrome
UniProt Protein Details:
NCBI Summary:	This gene encodes a member of the family of secreted protein kinases. The encoded protein binds calcium and phosphorylates proteins involved in bone mineralization. Mutations in this gene are associated with the autosomal recessive disorder Raine syndrome. [provided by RefSeq, Apr 2014]
UniProt Code:	Q8IXL6
NCBI GenInfo Identifier:	327478506
NCBI Gene ID:	56975
NCBI Accession:	Q8IXL6.2
UniProt Secondary Accession:	Q8IXL6,Q5I0W9, Q7Z4I0, Q9NPT2, A4D2Q5, L8B5W8
UniProt Related Accession:	Q8IXL6
Molecular Weight:	29,597 Da
NCBI Full Name:	Extracellular serine/threonine protein kinase FAM20C
NCBI Synonym Full Names:	family with sequence similarity 20 member C
NCBI Official Symbol:	FAM20C
NCBI Official Synonym Symbols:	RNS; DMP4; DMP-4; GEF-CK
NCBI Protein Information:	extracellular serine/threonine protein kinase FAM20C
UniProt Protein Name:	Extracellular serine/threonine protein kinase FAM20C
UniProt Synonym Protein Names:	Dentin matrix protein 4
Protein Family:	Extracellular serine/threonine protein kinase
UniProt Gene Name:	FAM20C
UniProt Entry Name:	FA20C_HUMAN