Cell Biology Assays

Ethanol Test Kit (BA0039)

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SKU:
BA0039
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine, Alcoholic Beverages
Research Area:
Oxidative Stress
Research Area:
Reactive Oxygen Species
Research Area:
Plant & Environmental Stress
Research Area:
Clinical Chemistry
Research Area:
Food Safety & Analysis

Description

ELISA Kit Technical ManualMSDS

Ethanol Test Kit - Information

This colorimetric ethanol test kit is based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr3+) product. The intensity of color, measured at 580 nm, is a direct measure of the alcohol concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.

Applications

For quantitative determination of ethanol and alcohol metabolism.

Ethanol Test Kit - Key Features

  • Sensitive and accurate. Detection range 0.04 - 4% alcohol in 96-well plate assay.
  • Convenient and high-throughput. The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
  • Versatility. Assays can be executed in 96-well plate or cuvet.

Ethanol Test Kit - Data Sheet

Kit IncludesReagent A: 50 mL Reagent B: 50 mL 10% TCA: 50 mL Standard: 2 mL 10% (v/v) ethanol
Kit RequiresPipeting (multi-channel) devices.
Method of DetectionOD580nm (Chemical)
Detection Limit0.04%
SamplesAlcoholic beverages etc
SpeciesAll
Protocol Length10 min
Size500 tests
StorageStore reagents at room temperature and the ethanol standard at 4°C.
Shelf Life12 months

More Details

Alcoholic drinksare among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C2H5OH) finds applications in basic research, drug discovery, clinic studies and winery. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable.
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