Antibody Name:	ESRP2 Antibody (PACO56326)
Antibody SKU:	PACO56326
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Epithelial splicing regulatory protein 2 protein (615-720AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate. All lanes: ESRP2 antibody at 6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 79, 78 kDa. Observed band size: 79 kDa.
	IHC image of PACO56326 diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	IHC image of PACO56326 diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Synonyms:

Epithelial splicing regulatory protein 2 (RNA-binding motif protein 35B) (RNA-binding protein 35B), ESRP2, RBM35B

UniProt Protein Function:	RBM35B: mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. Belongs to the ESRP family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: RNA-bindingChromosomal Location of Human Ortholog: 16q22.1Cellular Component: nucleoplasm; nucleusMolecular Function: mRNA binding; nucleotide bindingBiological Process: regulation of RNA splicing; RNA splicing; mRNA processing
UniProt Protein Details:
NCBI Summary:	ESPR2 is an epithelial cell-type-specific splicing regulator (Warzecha et al., 2009 [PubMed 19285943]).[supplied by OMIM, Aug 2009]
UniProt Code:	Q9H6T0
NCBI GenInfo Identifier:	45935393
NCBI Gene ID:	80004
NCBI Accession:	NP_079215
UniProt Secondary Accession:	Q9H6T0,Q8N6H8, Q8WZ15, Q9H6I4
UniProt Related Accession:	Q9H6T0
Molecular Weight:	77,364 Da
NCBI Full Name:	epithelial splicing regulatory protein 2
NCBI Synonym Full Names:	epithelial splicing regulatory protein 2
NCBI Official Symbol:	ESRP2
NCBI Official Synonym Symbols:	RBM35B
NCBI Protein Information:	epithelial splicing regulatory protein 2; RNA-binding protein 35B; RNA binding motif protein 35A; RNA binding motif protein 35B; RNA-binding motif protein 35B
UniProt Protein Name:	Epithelial splicing regulatory protein 2
UniProt Synonym Protein Names:	RNA-binding motif protein 35B; RNA-binding protein 35B
Protein Family:	Epithelial splicing regulatory protein
UniProt Gene Name:	ESRP2
UniProt Entry Name:	ESRP2_HUMAN