Antibody Name:	ERN2 Antibody (PACO19618)
Antibody SKU:	PACO19618
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, IHC
Recommended Dilutions:	ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:	Human
Immunogen:	Synthetic peptide of human ERN2

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using PACO19618(ERN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).
	The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO19618(ERN2 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

The accumulation of unfolded proteins within the endoplasmic recticulum (ER) of yeast and mammalian cells activates the unfolded protein response (UPR) pathway and leads to the transcription of ER-specific genes involved in protein folding. The activation of the UPR requires the ER transmembrane kinase IRE1p (for inositol-requiring and ER-to-nucleus signaling protein). IRE1 α and IRE1 β are two mammalian homologs of the yeast IRE1p. These related proteins localize to the ER lumen and contain both a short transmembrane domain that spans the ER membrane and a cytosolic Ser/Thr kinase domain. IRE1 activation involves the oligomerization and trans-phosphorylation of the cytosolic portion of the proteins, which then potentiates its intrinsic kinase activity and, in turn, stimulates transcription of UPR-targeted genes.

Synonyms:

endoplasmic reticulum to nucleus signaling 2

UniProt Protein Function:	IRE2: Induces translational repression through 28S ribosomal RNA cleavage in response to ER stress. Pro-apoptotic. Appears to play no role in the unfolded-protein response, unlike closely related proteins. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Apoptosis; Protein kinase, Other; Membrane protein, integral; Ribonuclease; Other group; IRE familyChromosomal Location of Human Ortholog: 16p12.2Molecular Function: ATP binding; endonuclease activity; magnesium ion binding; protein serine/threonine kinase activityBiological Process: apoptotic chromosome condensation; negative regulation of transcription, DNA-dependent; protein amino acid phosphorylation; rRNA catabolic process
UniProt Protein Details:
NCBI Summary:
UniProt Code:	Q76MJ5
NCBI GenInfo Identifier:	327478597
NCBI Gene ID:	10595
NCBI Accession:	Q76MJ5.4
UniProt Secondary Accession:	Q76MJ5,Q6ZNC0
UniProt Related Accession:	Q76MJ5
Molecular Weight:	102,480 Da
NCBI Full Name:	Serine/threonine-protein kinase/endoribonuclease IRE2
NCBI Synonym Full Names:	endoplasmic reticulum to nucleus signaling 2
NCBI Official Symbol:	ERN2
NCBI Official Synonym Symbols:	IRE1b; IRE2p; hIRE2p; IRE1-BETA
NCBI Protein Information:	serine/threonine-protein kinase/endoribonuclease IRE2
UniProt Protein Name:	Serine/threonine-protein kinase/endoribonuclease IRE2
UniProt Synonym Protein Names:	Endoplasmic reticulum-to-nucleus signaling 2; Inositol-requiring protein 2; hIRE2p; Ire1-beta; IRE1b
Protein Family:
UniProt Gene Name:	ERN2
UniProt Entry Name:	ERN2_HUMAN