Equine Cytokine Array (10 targets) (SARB0013)

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SKU:
SARB0013
Product Type:
Protein Array
Analytes:
IFN-gamma
Analytes:
IL-1 alpha (IL-1 F1)
Analytes:
IL-1 Ra (IL-1 F3)
Analytes:
IL-2
Analytes:
IL-4
Analytes:
IL-8 (CXCL8)
Analytes:
IL-10
Analytes:
IL-15
Analytes:
MCP-1 (CCL2)
Analytes:
VEGF-A
Reactivity:
Equine
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0013
Size:2, 4, or 8
Species Detected:Equine
Number of Targets Detected:10
Gene Symbols:CCL2, CXCL8, IFNG, IL10, IL1A, IL1RA, IL1RN, IL2, IL4, IL8|MCP1, SCYA2, VEGF, VEGFA
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 100034181
  • Accession #: P42160
IL-1 alpha (IL-1 F1)
  • Gene Symbol: IL1A
  • Gene ID: 100064969
  • Accession #: Q28385
IL-1 Ra (IL-1 F3)
  • Gene Symbol: IL1RN IL1RA
  • Gene ID: 100034236
  • Accession #: O18999
IL-2
  • Gene Symbol: IL2
  • Gene ID: 100034204
  • Accession #: P37997
IL-4
  • Gene Symbol: IL4
  • Gene ID: 100034225
  • Accession #: P42202
IL-8 (CXCL8)
  • Gene Symbol: CXCL8 IL8
  • Gene ID: 100037400
  • Accession #: O62812
IL-10
  • Gene Symbol: IL10
  • Gene ID: 100034187
  • Accession #: Q28374
IL-15
  • Gene Symbol:
  • Gene ID:
  • Accession #:
MCP-1 (CCL2)
  • Gene Symbol: CCL2 MCP1 SCYA2
  • Gene ID: 100034136
  • Accession #: Q9TTQ3
VEGF-A
  • Gene Symbol: VEGFA VEGF
  • Gene ID: 100033839
  • Accession #: Q9GKR0
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Equine (Horse) Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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