Name: ELP4 Antibody (PACO59337)
SKU: PACO59337
Availability: OutOfStock

Antibody Name:	ELP4 Antibody (PACO59337)
Antibody SKU:	PACO59337
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:200-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human Elongator complex protein 4 protein (117-223AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	IHC image of PACO59337 diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
	Immunofluorescence staining of MCF-7 cells with PACO59337 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	IHC image of PACO59337 diluted at 1:600 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
Synonyms:	Elongator complex protein 4 (hELP4) (PAX6 neighbor gene protein), ELP4, C11orf19 PAXNEB

UniProt Protein Function:	ELP4: Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. Belongs to the ELP4 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Unknown functionChromosomal Location of Human Ortholog: 11p13Cellular Component: nucleoplasm; transcription elongation factor complex; Elongator holoenzyme complex; cytoplasm; histone acetyltransferase complexMolecular Function: protein binding; histone acetyltransferase activity; phosphorylase kinase regulator activityBiological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; RNA elongation from RNA polymerase II promoter; regulation of protein kinase activityDisease: Aniridia
UniProt Protein Details:
NCBI Summary:	This gene encodes a component of the six subunit elongator complex, a histone acetyltransferase complex that associates directly with RNA polymerase II during transcriptional elongation. The human gene can partially complement sensitivity phenotypes of yeast ELP4 deletion mutants. This gene has also been associated with Rolandic epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
UniProt Code:	Q96EB1
NCBI GenInfo Identifier:	570359562
NCBI Gene ID:	26610
NCBI Accession:	NP_001275654.1
UniProt Secondary Accession:	Q96EB1,Q9H4E8, Q9NX11, B4E3W0, E7EPZ6
UniProt Related Accession:	Q96EB1
Molecular Weight:	52,765 Da
NCBI Full Name:	elongator complex protein 4 isoform 2
NCBI Synonym Full Names:	elongator acetyltransferase complex subunit 4
NCBI Official Symbol:	ELP4
NCBI Official Synonym Symbols:	AN; hELP4; PAXNEB; PAX6NEB; C11orf19; dJ68P15A.1
NCBI Protein Information:	elongator complex protein 4
UniProt Protein Name:	Elongator complex protein 4
UniProt Synonym Protein Names:	PAX6 neighbor gene protein
Protein Family:	Elongator complex protein
UniProt Gene Name:	ELP4
UniProt Entry Name:	ELP4_HUMAN