EFHD1 Antibody (PACO61678)
- SKU:
- PACO61678
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | EFHD1 Antibody (PACO61678) |
| Antibody SKU: | PACO61678 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human EF-hand domain-containing protein D1 protein (15-72AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate. All lanes: EFHD1 antibody at 3.4µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 27, 17 kDa. Observed band size: 27 kDa. |
 | Immunofluorescence staining of A549 cells with PACO61678 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO61678 diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production. May play a role in neuronal differentiation (By similarity). |
| Synonyms: | EF-hand domain-containing protein D1, EF-hand domain-containing protein 1, Swiprosin-2, EFHD1, SWS2, PP3051 |
| UniProt Protein Function: | EFHD1: 2 isoforms of the human protein are produced by alternative splicingProtein type: MitochondrialChromosomal Location of Human Ortholog: 2q37.1 |
| UniProt Protein Details: | |
| NCBI Summary: | EFHD1 is an EF-hand domain-containing protein that displays increased expression during neuronal differentiation (Tominaga and Tomooka, 2002 [PubMed 12270117]).[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9BUP0 |
| NCBI GenInfo Identifier: | 20140222 |
| NCBI Gene ID: | 80303 |
| NCBI Accession: | Q9BUP0.1 |
| UniProt Secondary Accession: | Q9BUP0,Q9BTF8, Q9H8I2, Q9HBQ0, B2RD83, E9PFH3 |
| UniProt Related Accession: | Q9BUP0 |
| Molecular Weight: | 16,145 Da |
| NCBI Full Name: | EF-hand domain-containing protein D1 |
| NCBI Synonym Full Names: | EF-hand domain family member D1 |
| NCBI Official Symbol: | EFHD1 |
| NCBI Official Synonym Symbols: | SWS2; MST133; PP3051; MSTP133 |
| NCBI Protein Information: | EF-hand domain-containing protein D1 |
| UniProt Protein Name: | EF-hand domain-containing protein D1 |
| UniProt Synonym Protein Names: | EF-hand domain-containing protein 1; Swiprosin-2 |
| Protein Family: | EF-hand domain-containing protein |
| UniProt Gene Name: | EFHD1 |
| UniProt Entry Name: | EFHD1_HUMAN |
| Antibodies |
| EFHD1 Antibody (PACO00089) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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