Microbiology Recombinant Proteins
E.coli Tryptophan Synthase Recombinant Protein (RPES3431)
- SKU:
- RPES3431
Description
E.coli Tryptophan Synthase Recombinant Protein
Tryptophan synthase is a multienzyme ?2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp). The ?-subunit catalyzes cleavage of 3-indole-d-glycerol 3?-phosphate (IGP) to give indole and D-glyceraldehyde 3?-phosphate (G3P). Indole is then transferred through a 25-? hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.
| Product Name: | E.coli Tryptophan Synthase Recombinant Protein (RPES3431) |
| Product Code: | RPES3431 |
| Size: | 10µg |
| Species: | E.coli |
| Expressed Host: | E.coli |
| Synonyms: | Tryptophan synthetase, Tryptophan synthase |
| Accession: | P0A877&P0A879 |
| Sequence: | Met1-Ser268&Thr2-Ile397 |
| Fusion tag: | N-His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant E.coli Tryptophan synthase is produced by our E.coli expression system and the target gene encoding Met1-Ser268&Thr2-Ile397 is expressed with a 6His tag at the N-terminus. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 72.5 kDa |
| AP Mol Mass: | 28&40-50 kDa |
| Formulation: | Supplied as a 0.2 µm filtered solution of PBS, pH7.4. |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Escherichia coli |
Research Area: |
Microbiology |
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