Microbiology Recombinant Proteins
E.coli Trp B Recombinant Protein (RPES3392)
- SKU:
- RPES3392
Description
E.coli Trp B Recombinant Protein
Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an ?-ββ-? complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.
| Product Name: | E.coli Trp B Recombinant Protein (RPES3392) |
| Product Code: | RPES3392 |
| Size: | 10µg |
| Species: | E.coli |
| Expressed Host: | E.coli |
| Synonyms: | Tryptophan synthase beta chain, trpB |
| Accession: | P0A879 |
| Sequence: | Thr2-Ile397 |
| Fusion tag: | N-His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant E.coli Tryptophan synthase beta chain is produced by our E.coli expression system and the target gene encoding Thr2-Ile397 is expressed with a 6His tag at the N-terminus. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 43.8 kDa |
| AP Mol Mass: | 40-50 kDa |
| Formulation: | Supplied as a 0.2 µm filtered solution of PBS, pH7.4. |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Escherichia coli |
Research Area: |
Microbiology |
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