Microbiology Recombinant Proteins

E.Coli SURA Recombinant Protein (RPPB2300)

(No reviews yet) Write a Review
SKU:
RPPB2300
Product Type:
Recombinant Protein
Species:
Escherichia coli
Uniprot:
P0ABZ6
Research Area:
Microbiology

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:E.Coli SURA Recombinant Protein
Product Code:RPPB2300
Size:20µg
Species:E.Coli
Target:SURA
Synonyms:Rotamase surA, Survival protein A.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The SURA 1mg/ml protein solution contains 20mM Tris-HCl, pH-8, and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN
Biological Activity:Specific activity is > 450 nmoles/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.

SURA is a PPIase enzyme and chaperone of Escherichia coli and other Gram-negative bacteria. SURA is a key player in the biogenesis of beta-barrel outer membrane proteins and is involved in cell envelope homeostasis and cell envelope functions. SURA is necessary for the survival of E.coli in stationary phase and needed for pilus biogenesis.

SURA E.Coli Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 429 amino acids (21-428 a.a.) and having a molecular weight of 47.3kDa. The SURA is fused to 20 a.a His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:Chaperone involved in the correct folding and assembly of outer membrane proteins, such as OmpA, OmpF and LamB. Recognizes specific patterns of aromatic residues and the orientation of their side chains, which are found more frequently in integral outer membrane proteins. May act in both early periplasmic and late outer membrane-associated steps of protein maturation. Essential for the survival of E.coli in stationary phase. Required for pilus biogenesis.
UniProt Protein Details:
NCBI Summary:Parvulin (ppiC) family. [More information is available at EcoGene: EG10985]. SurA in Escherichia coli is a periplasmic peptidyl-prolyl isomerase which is necessary for the proper folding of outer membrane proteins including OmpA, OmpF and LamB. [More information is available at EcoCyc: EG10985].
UniProt Code:P0ABZ6
NCBI GenInfo Identifier:16128047
NCBI Gene ID:944812
NCBI Accession:NP_414595
UniProt Secondary Accession:P0ABZ6,P21202, P75630, Q8KIP6, Q8KMY0
UniProt Related Accession:P0ABZ6
Molecular Weight:47.3 kDa (429aa), confirmed by MALDI-TOF
NCBI Full Name:peptidyl-prolyl cis-trans isomerase (PPIase)
NCBI Synonym Full Names:
NCBI Official Symbol:surA��
NCBI Official Synonym Symbols:ECK0054; JW0052��
NCBI Protein Information:peptidyl-prolyl cis-trans isomerase (PPIase)
UniProt Protein Name:Chaperone SurA
UniProt Synonym Protein Names:Peptidyl-prolyl cis-trans isomerase SurA (EC:5.2.1.8); PPIase SurA; Rotamase SurA; Survival protein A
Protein Family:Chaperone
UniProt Gene Name:surA��
UniProt Entry Name:
View AllClose

0 Reviews

View AllClose

Related Products