Microbiology Recombinant Proteins

E.Coli SlyD Recombinant Protein (RPPB2275)

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SKU:
RPPB2275
Product Type:
Recombinant Protein
Species:
Escherichia coli
Uniprot:
P0A9K9
Research Area:
Microbiology

Description

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Product Name:E.Coli SlyD Recombinant Protein
Product Code:RPPB2275
Size:20µg
Species:E.Coli
Target:SlyD
Synonyms:FKBP-Type Peptidyl-Prolyl Cis-Trans Isomerase, SlyD.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:SlyD protein solution contains 20mM Tris pH-7.5.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
Biological Activity:Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
UniProt Code:P0A9K9

SlyD accessiton#: NP_755987 is a putative folding helper protein from the Escherichia coli cytosol, which has N-terminal prolyl isomerase domain of the FKBP type and a most likely unstructured C-terminal tail. SlyD is an important factor in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.

SlyD Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids and having a molecular mass of 21 kDa.

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