Microbiology Recombinant Proteins
E.Coli NTH Recombinant Protein (RPPB2041)
- SKU:
- RPPB2041
- Product Type:
- Recombinant Protein
- Species:
- Escherichia coli
- Uniprot:
- P0AB83
- Research Area:
- Microbiology
Description
| Product Name: | E.Coli NTH Recombinant Protein |
| Product Code: | RPPB2041 |
| Size: | 10µg |
| Species: | E.Coli |
| Target: | NTH |
| Synonyms: | DNA-(apurinic or apyrimidinic site) lyase, b1633, JW1625. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The NTH solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT, 0.1mM PMSF and 40% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE� EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I |
| UniProt Code: | P0AB83 |
Endonuclease III (nth) is a DNA repair enzyme which has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases numerous damaged pyrimidines from DNA by cleaving the N-glycosidic bond and leaving an AP (apurinic/apyrimidinic) site. This AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, thus leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
NTH E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 25.7kDa. The NTH is purified by proprietary chromatographic techniques.
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