Description
| Product Name: | E.coli Lactose Operon Repressor/LacI Recombinant Protein (His Tag) |
| Product Code: | RPES5811 |
| Size: | 20µg |
| Species: | E.coli |
| Expression Host: | E.coli |
| Synonyms: | Lactose operon repressor, LacI |
| Mol Mass: | 39.4 kDa |
| AP Mol Mass: | 40 kDa |
| Tag: | C-His |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin Level: | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Bio Activity: | Testing in progress |
| Sequence: | Met1-Gln360 |
| Accession: | P03023 |
| Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation: | Supplied as a 0.2 μm filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printe |
| Reconstitution: | Not Applicable |
| Background: | Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity. |
