Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
Product Name:
E.coli LacI Recombinant Protein (RPES3452)
Product Code:
RPES3452
Size:
10µg
Species:
E.coli
Expressed Host:
E.coli
Synonyms:
Lactose operon repressor, LacI
Accession:
P03023-1
Sequence:
Met1-Gln360
Fusion tag:
C-His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant E.coli Lactose operon repressor is produced by our E.coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
39.4 kDa
AP Mol Mass:
40 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
