Microbiology Recombinant Proteins
E.coli LacI Recombinant Protein (RPES3452)
- SKU:
- RPES3452
- Product Type:
- Recombinant Protein
- Species:
- Escherichia coli
- Research Area:
- Microbiology
Description
E.coli LacI Recombinant Protein
Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
| Product Name: | E.coli LacI Recombinant Protein (RPES3452) |
| Product Code: | RPES3452 |
| Size: | 10µg |
| Species: | E.coli |
| Expressed Host: | E.coli |
| Synonyms: | Lactose operon repressor, LacI |
| Accession: | P03023-1 |
| Sequence: | Met1-Gln360 |
| Fusion tag: | C-His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant E.coli Lactose operon repressor is produced by our E.coli expression system and the target gene encoding Met1-Gln360 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 39.4 kDa |
| AP Mol Mass: | 40 kDa |
| Formulation: | Supplied as a 0.2 µm filtered solution of 20mM Tris, 300mM NaCl, 5mM DTT pH 8.0. |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20°C. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
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