Microbiology Recombinant Proteins

E.Coli IDNK Recombinant Protein (RPPB2551)

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SKU:
RPPB2551
Product Type:
Recombinant Protein
Species:
Escherichia coli
Uniprot:
P39208
Research Area:
Microbiology

Description

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Product Name:E.Coli IDNK Recombinant Protein
Product Code:RPPB2551
Size:20µg
Species:E.Coli
Target:IDNK
Synonyms:Thermosensitive gluconokinase, Gluconate kinase 1, idnK.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:IDNK protein solution (1mg/ml) containing 20mM Tris-HClbuffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMSQGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQRRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIANVTEQCRQAVL AIRQNRICAK EGSASDQRCE
UniProt Code:P39208

Thermosensitive Gluconokinase, also known as IDNK is a 187 a.a protein whichis a member of the gluconokinase gntK/gntV family. IDNK catalyzes theconversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. Inaddition, IDNK is considered to take part in gender determination, deletion ofthe distal portion of 9p is able to lead to a development of male to female sexreversal, the phenotype of a female with a male X, Y genotype.

IDNK E.Coli Recombinant produced in E.Coli is a single,non-glycosylated polypeptide chain containing 210 amino acids (1-187 a.a) andhaving a molecular mass of 23.4kDa.IDNK is fused to a 23 amino acid His-tag at N-terminus& purified by proprietary chromatographic techniques.

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