Microbiology Recombinant Proteins

E.Coli GLSA1 Recombinant Protein (RPPB1705)

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SKU:
RPPB1705
Product Type:
Recombinant Protein
Species:
Escherichia coli
Uniprot:
P77454
Research Area:
Microbiology

Description

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Product Name:E.Coli GLSA1 Recombinant Protein
Product Code:RPPB1705
Size:20µg
Species:E.Coli
Target:GLSA1
Synonyms:Glutaminase 1, ybaS.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The GLSA1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG

GlsA1 is a member of the great superfamily of serine-dependent beta-lactamases and penicillin-binding proteins which exist almost in every bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+.

GLSA1 E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 330 amino acids (1-310a.a.) and having a molecular mass of 35.0kDa. The GLSA1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:Two glutaminases have been purified and biochemically characterized from Escherichia coli B. ybaS might contribute to acid resistance (Brown, 2008). [More information is available at EcoGene: EG13247]. YbaS is a glutaminase that is highly selective for L-glutamine. [More information is available at EcoCyc: G6261].
UniProt Code:P77454
NCBI GenInfo Identifier:16128469
NCBI Gene ID:946187
NCBI Accession:NP_415018.1
UniProt Secondary Accession:P77454,Q2MBU2
UniProt Related Accession:P77454
Molecular Weight:32,903 Da
NCBI Full Name:glutaminase 1
NCBI Synonym Full Names:
NCBI Official Symbol:glsA��
NCBI Official Synonym Symbols:ECK0479; glsA1; JW0474; ybaS��
NCBI Protein Information:glutaminase 1
UniProt Protein Name:Glutaminase 1
UniProt Synonym Protein Names:
Protein Family:Glutaminase
UniProt Gene Name:glsA1��
UniProt Entry Name:GLSA1_ECOLI
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