Microbiology Recombinant Proteins
E.Coli glpE Recombinant Protein (RPPB1697)
- SKU:
- RPPB1697
- Product Type:
- Recombinant Protein
- Species:
- Escherichia coli
- Uniprot:
- P0A6V5
- Research Area:
- Microbiology
Description
Product Name: | E.Coli glpE Recombinant Protein |
Product Code: | RPPB1697 |
Size: | 20µg |
Species: | E.Coli |
Target: | glpE |
Synonyms: | ECK3411, JW3388, b3425, Thiosulfate sulfurtransferase GlpE. |
Source: | Escherichia Coli |
Physical Appearance: | Sterile Filtered colorless solution. |
Formulation: | The glpE solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
Purity: | Greater than 95.0% as determined by SDS-PAGE. |
Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMDQFECI NVADAHQKLQ EKEAVLVDIR DPQSFAMGHA VQAFHLTNDT LGAFMRDNDF DTPVMVMCYH GNSSKGAAQY LLQQGYDVVY SIDGGFEAWQ RQFPAEVAYG A |
UniProt Code: | P0A6V5 |
Thiosulfate sulfurtransferase (glpE) is a mitochondrial matrix enzyme which is encoded by the nucleus. Escherichia coli glpE is a prototype for the single-domain rhodanese superfamily. glpE catalyzes the sulfur-transfer reaction in which a sulfur atom is transferred from thiosulfate to cyanide by a double-displacement mechanism.
glpE Recombinant produced in E. coli is a single polypeptide chain containing 131 amino acids (1-108) and having a molecular mass of 14.5kDa. glpE is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.