Microbiology Recombinant Proteins

E.Coli FIMC Recombinant Protein (RPPB3544)

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SKU:
RPPB3544
Product Type:
Recombinant Protein
Species:
Escherichia coli
Uniprot:
P31697
Research Area:
Microbiology

Description

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Product Name:E.Coli FIMC Recombinant Protein
Product Code:RPPB3544
Size:25µg
Species:E.Coli
Target:FIMC
Synonyms:Chaperone protein fimC, fimC, b4316, JW4279.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The FIMC solution (1 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGVALGATRV IYPAGQKQEQ LAVTNNDENS TYLIQSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLFWMNVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLINPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME
UniProt Code:P31697

The 23kDa two-domain periplasmic chaperone FIMC from E.coli is vital for the assembly of type-1 pili, which are filamentous, very oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages.

FIMC E.Coli Recombinant fused with a�21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�226 amino acids (37-241 a.a.) and having a molecular mass of 25kDa. The FIMC is purified by proprietary chromatographic techniques.

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