Microbiology Recombinant Proteins

E.Coli DsbE Recombinant Protein (RPPB5217)

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SKU:
RPPB5217
Product Type:
Recombinant Protein
Species:
Escherichia coli
Uniprot:
P0AA86
Research Area:
Microbiology

Description

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Product Name:E.Coli DsbE Recombinant Protein
Product Code:RPPB5217
Size:25µg
Species:E.Coli
Target:DsbE
Synonyms:Thiol:disulfide interchange protein dsbE, Cytochrome c biogenesis protein ccmG, dsbE, ccmG, yejQ, b2195, JW2183.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The DsbE protein solution contains 20mM Tris-HCl, pH-7.5, 2mM EDTA and 10% Glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MRNAEGDDPT NLESALIGKP VPKFRLESLD NPGQFYQADV LTQGKPVLLN VWATWCPTCR AEHQYLNQLS AQGIRVVGMN YKDDRQKAIS WLKELGNPYA LSLFDGDGML GLDLGVYGAP ETFLIDGNGI IRYRHAGDLN PRVWEEEIKP LWEKYSKEAA Q
UniProt Code:P0AA86

DsbE is a reducing Dsb protein involved in electron transfer for cytochrome c maturation in the periplasm of Escherichia coli. DsbE is one of 12 proteins required for their assembly in the periplasm. DsbE functions is to decrease disulphide bonds formed among correctly paired cysteine residues in the cytochrome c apoproteins prior to haem attachment by CcmF and CcmH.

Recombinant DsbE produced in E.Coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18.1 kDa. DsbE is purified by conventional chromatography techniques.

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