DTX3 Antibody (PACO63179)
- SKU:
- PACO63179
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | DTX3 Antibody (PACO63179) |
| Antibody SKU: | PACO63179 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Probable E3 ubiquitin-protein ligase DTX3 protein (90-347AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO63179 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of SH-SY5Y cells with PACO63179 at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context (By similarity). Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. |
| Synonyms: | Probable E3 ubiquitin-protein ligase DTX3 (EC 2.3.2.27) (Protein deltex-3) (Deltex3) (RING finger protein 154) (RING-type E3 ubiquitin transferase DTX3), DTX3, RNF154 |
| UniProt Protein Function: | DTX3: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context. Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. Belongs to the Deltex family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-Chromosomal Location of Human Ortholog: 12q13.3Molecular Function: protein binding |
| UniProt Protein Details: | |
| NCBI Summary: | DTX3 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]).[supplied by OMIM, Nov 2009] |
| UniProt Code: | Q8N9I9 |
| NCBI GenInfo Identifier: | 37077338 |
| NCBI Gene ID: | 196403 |
| NCBI Accession: | Q8N9I9.2 |
| UniProt Secondary Accession: | Q8N9I9,Q53ZZ2, Q8NAU6, Q8NDS8 |
| UniProt Related Accession: | Q8N9I9 |
| Molecular Weight: | 38,155 Da |
| NCBI Full Name: | Probable E3 ubiquitin-protein ligase DTX3 |
| NCBI Synonym Full Names: | deltex E3 ubiquitin ligase 3 |
| NCBI Official Symbol: | DTX3 |
| NCBI Official Synonym Symbols: | RNF154; deltex3 |
| NCBI Protein Information: | probable E3 ubiquitin-protein ligase DTX3 |
| UniProt Protein Name: | Probable E3 ubiquitin-protein ligase DTX3 |
| UniProt Synonym Protein Names: | Protein deltex-3; Deltex3; RING finger protein 154 |
| Protein Family: | Protein DETOXIFICATION |
| UniProt Gene Name: | DTX3 |
| UniProt Entry Name: | DTX3_HUMAN |
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