DPPA3 Antibody (PACO57224)
- SKU:
- PACO57224
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | DPPA3 Antibody (PACO57224) |
| Antibody SKU: | PACO57224 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Developmental pluripotency-associated protein 3 protein (1-159AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO57224 diluted at 1:400 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis (By similarity). |
| Synonyms: | Developmental pluripotency-associated protein 3 (Stella-related protein), DPPA3, STELLAR |
| UniProt Protein Function: | DPPA3: Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation. Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark. Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis. May be important for the totipotent/pluripotent states continuing through preimplantation development. Also involved in chromatin condensation in oocytogenesis.Chromosomal Location of Human Ortholog: 12p13.31Cellular Component: cytoplasm; female pronucleus; male pronucleus; nucleusMolecular Function: methylated histone residue binding |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a protein that in mice may function as a maternal factor during the preimplantation stage of development. In mice, this gene may play a role in transcriptional repression, cell division, and maintenance of cell pluripotentiality. In humans, related intronless loci are located on chromosomes 14 and X. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q6W0C5 |
| NCBI GenInfo Identifier: | 74749483 |
| NCBI Gene ID: | 359787 |
| NCBI Accession: | Q6W0C5.1 |
| UniProt Secondary Accession: | Q6W0C5,Q0P5U3, Q6JZS6 |
| UniProt Related Accession: | Q6W0C5 |
| Molecular Weight: | 17,851 Da |
| NCBI Full Name: | Developmental pluripotency-associated protein 3 |
| NCBI Synonym Full Names: | developmental pluripotency associated 3 |
| NCBI Official Symbol: | DPPA3 |
| NCBI Official Synonym Symbols: | STELLA |
| NCBI Protein Information: | developmental pluripotency-associated protein 3 |
| UniProt Protein Name: | Developmental pluripotency-associated protein 3 |
| UniProt Synonym Protein Names: | Stella-related protein |
| Protein Family: | Developmental pluripotency-associated protein |
| UniProt Gene Name: | DPPA3 |
| UniProt Entry Name: |
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