DPP4 Activity Fluorometric Assay Kit

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SKU:
MAES0237
Product Type:
Assay
Instrument:
Fluorescence Microplate Reader
Detection Method:
Fluorometric
Sample Type:
Serum, Plasma, Tissue And Cell
Sensitivity:
0.92 U/L
Range:
0.92-3.77 U/L
Assay Time:
70 min

Description

Product Name:DPP4 Activity Fluorometric Assay Kit
Product Code:MAES0237
Sample Type:Serum, Plasma, Tissue And Cell
Assay Time:70 min
Assay Type:Enzyme Activity
Synonyms:DPP4
Detection Instrument:Fluorescence microplate reader (Ex/Em=360 nm/460 nm)
Detection Method:Fluorescence method
Sensitivity:0.92 U/L
Detection Range:0.92-3.77 U/L
Inter-assay CV:0.057
Intra-assay CV:0.006

Dipeptidyl Peptidase IV (DPP4), also known as CD26, is a kind of serine protease, can decompose the second N-terminal proline or alanine residue of the peptide chain. In organisms, dipeptidyl peptidase can rapidly decompose incretin, which can stabilize insulin level and promote the reduction of blood glucose level in organisms. The detection principle of this kit is that DPP4 can decompose the substrate and release the fluorescent substance AMC, and the activity of this enzyme can be inhibited by adding DPP4 inhibitor. The activity of DPP4 could be calculated by the difference of fluorescence value before and after inhibition.

Storage:-20°C
Valid Period:12 months
Other Instruments Required:Vortex mixer, 37℃incubator, Centrifuge
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