Description
Antibody Name: | DPH6 Antibody (PACO36926) |
Antibody SKU: | PACO36926 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Diphthine--ammonia ligase protein (8-267AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
Immunohistochemistry of paraffin-embedded human kidney tissue using PACO36926 at dilution of 1:100. | |
Immunofluorescent analysis of HepG2 cells using PACO36926 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor (EEF2) to diphthamide. |
Synonyms: | Diphthine--ammonia ligase (EC 6.3.1.14) (ATP-binding domain-containing protein 4) (Diphthamide synthase) (Diphthamide synthetase) (Protein DPH6 homolog), DPH6, ATPBD4 |
UniProt Protein Function: | DPH6: Amidase that catalyzes the last step of diphthamide biosynthesis using ammonium and ATP. Diphthamide biosynthesis consists in the conversion of an L-histidine residue in the translation elongation factor (EEF2) to diphthamide. Belongs to the Diphthine--ammonia ligase family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: EC 6.3.1.14Chromosomal Location of Human Ortholog: 15q14Cellular Component: cytosol; nucleolus; nucleusMolecular Function: ATP binding; diphthine-ammonia ligase activityBiological Process: cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; post-translational protein modification; regulation of protein stability |
UniProt Protein Details: | |
NCBI Summary: | |
UniProt Code: | Q7L8W6 |
NCBI GenInfo Identifier: | 213972615 |
NCBI Gene ID: | 89978 |
NCBI Accession: | NP_001135444.1 |
UniProt Secondary Accession: | Q7L8W6,Q96HJ6, B3KWG1 |
UniProt Related Accession: | Q7L8W6 |
Molecular Weight: | 18,657 Da |
NCBI Full Name: | diphthine--ammonia ligase isoform 2 |
NCBI Synonym Full Names: | diphthamine biosynthesis 6 |
NCBI Official Symbol: | DPH6 |
NCBI Official Synonym Symbols: | ATPBD4 |
NCBI Protein Information: | diphthine--ammonia ligase |
UniProt Protein Name: | Diphthine--ammonia ligase |
UniProt Synonym Protein Names: | ATP-binding domain-containing protein 4; Diphthamide synthase; Diphthamide synthetase; Protein DPH6 homolog |
Protein Family: | Diphthine--ammonia ligase |
UniProt Gene Name: | DPH6 |
UniProt Entry Name: | DPH6_HUMAN |