Description
Antibody Name: | DPH5 Antibody (PACO61722) |
Antibody SKU: | PACO61722 |
Size: | 50ul |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:500-1:1000, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human Diphthine methyl ester synthase protein (138-235AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
IHC image of PACO61722 diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. | |
Immunofluorescence staining of SH-SY5Y cells with PACO61722 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis. |
Synonyms: | Diphthine methyl ester synthase (EC 2.1.1.314) (Diphthamide biosynthesis methyltransferase), DPH5 |
UniProt Protein Function: | DPH5: S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis. Belongs to the diphthine synthase family. 6 isoforms of the human protein are produced by alternative splicing.Protein type: Methyltransferase; EC 2.1.1.98Chromosomal Location of Human Ortholog: 1p21.2Cellular Component: cytosolMolecular Function: diphthine synthase activityBiological Process: methylation; cellular protein metabolic process; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; post-translational protein modification |
UniProt Protein Details: | |
NCBI Summary: | This gene encodes a component of the diphthamide synthesis pathway. Diphthamide is a post-translationally modified histidine residue found only on translation elongation factor 2. It is conserved from archaebacteria to humans, and is targeted by diphtheria toxin and Pseudomonas exotoxin A to halt cellular protein synthesis. The yeast and Chinese hamster homologs of this protein catalyze the trimethylation of the histidine residue on elongation factor 2, resulting in a diphthine moiety that is subsequently amidated to yield diphthamide. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
UniProt Code: | Q9H2P9 |
NCBI GenInfo Identifier: | 117190326 |
NCBI Gene ID: | 51611 |
NCBI Accession: | NP_001070862.1 |
UniProt Secondary Accession: | Q9H2P9 |
UniProt Related Accession: | Q9H2P9 |
Molecular Weight: | 25 kDa |
NCBI Full Name: | diphthine methyl ester synthase isoform a |
NCBI Synonym Full Names: | diphthamide biosynthesis 5 |
NCBI Official Symbol: | DPH5 |
NCBI Official Synonym Symbols: | AD-018; CGI-30; NPD015; HSPC143 |
NCBI Protein Information: | diphthine methyl ester synthase |
UniProt Protein Name: | Diphthine synthase |
UniProt Synonym Protein Names: | Diphthamide biosynthesis methyltransferase |
Protein Family: | Diphthine methyl ester synthase |
UniProt Gene Name: | DPH5 |
UniProt Entry Name: | DPH5_HUMAN |