Canine ELISA Kits
Dog C-X-C motif chemokine 10 (CXCL10) ELISA Kit
- SKU:
- CNEB0082
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- Q5KSV9
- ELISA Type:
- Sandwich
- Reactivity:
- Canine
Description
Dog C-X-C motif chemokine 10 (CXCL10) ELISA Kit
Key Features
| Save Time | Pre-coated 96 well plate | |
| Quick Start | Kit includes all necessary reagents | |
| Publication Ready | Reproducible and reliable results |
Overview
| Product Name: | Dog C-X-C motif chemokine 10 (CXCL10) ELISA Kit |
| Product Code: | CNEB0082 |
| Alias: | C-X-C motif chemokine 10, Small-inducible cytokine B10, CXCL10 |
| Uniprot: | Q5KSV9 |
| Reactivity: | Dog |
| Range: | Please contact us for more information |
| Detection Method: | Sandwich |
| Size: | 96 Assays |
| Storage: | Please see kit components below for exact storage details |
| Note: | For Research Use Only |
Protein Information
| UniProt Protein Function: | Function: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3 By similarity.Subcellular location: Secreted By similarity. Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family. |
| UniProt Code: | |
| NCBI GenInfo Identifier: | |
| NCBI Gene ID: | |
| NCBI Accession: | |
| UniProt Related Accession: | |
| Molecular Weight: | 10.1kDa |
Kit Components
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8x12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10ml | -20°C |
| Detection Reagent A | 60µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer (25X) | 30ml | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10ml | 4°C |
| Plate Sealer | 5 | - |
Other materials required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
Protocol
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
| Step | Procedure |
| 1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
| 2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
| 3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
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