DNASE2 Antibody (PACO49530)
- SKU:
- PACO49530
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | DNASE2 Antibody (PACO49530) |
| Antibody SKU: | PACO49530 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Deoxyribonuclease-2-α protein (19-152AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate. All lanes: DNASE2 antibody at 2.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 34 kDa. Observed band size: 40 kDa.. |
 | IHC image of PACO49530 diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Hydrolyzes DNA under acid, c conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. |
| Synonyms: | Deoxyribonuclease-2-α (EC 3.1.22.1) (acid, DNase) (Deoxyribonuclease II alpha) (DNase II alpha) (Lysosomal DNase II) (R31240_2), DNASE2, DNASE2A DNL2 |
| UniProt Protein Function: | DNASE2: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. Belongs to the DNase II family.Protein type: EC 3.1.22.1; DeoxyribonucleaseChromosomal Location of Human Ortholog: 19p13.2Cellular Component: lysosome; intracellularMolecular Function: DNA binding; deoxyribonuclease II activityBiological Process: erythrocyte differentiation; DNA fragmentation during apoptosis; DNA metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | This gene encodes a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O00115 |
| NCBI GenInfo Identifier: | 3182984 |
| NCBI Gene ID: | 1777 |
| NCBI Accession: | O00115.2 |
| UniProt Secondary Accession: | O00115,O43910, B2RD06 |
| UniProt Related Accession: | O00115 |
| Molecular Weight: | 39,581 Da |
| NCBI Full Name: | Deoxyribonuclease-2-alpha |
| NCBI Synonym Full Names: | deoxyribonuclease II, lysosomal |
| NCBI Official Symbol: | DNASE2 |
| NCBI Official Synonym Symbols: | DNL; DNL2; DNASE2A |
| NCBI Protein Information: | deoxyribonuclease-2-alpha; R31240_2; acid DNase; DNase II alpha; lysosomal DNase II; DNase II, lysosomal; deoxyribonuclease II alpha |
| UniProt Protein Name: | Deoxyribonuclease-2-alpha |
| UniProt Synonym Protein Names: | Acid DNase; Deoxyribonuclease II alpha; DNase II alpha; Lysosomal DNase II; R31240_2 |
| Protein Family: | Deoxyribonuclease |
| UniProt Gene Name: | DNASE2 |
| UniProt Entry Name: | DNS2A_HUMAN |
| Antibodies | ELISA Kits |
| DNASE2 Antibody (PACO08900) | Human DNASE2 (Deoxyribonuclease-2-alpha) ELISA Kit (HUFI03469) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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