Description
Antibody Name: | DIRAS1 Antibody (PACO60685) |
Antibody SKU: | PACO60685 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, IHC, IF |
Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
Species Reactivity: | Human |
Immunogen: | Recombinant Human GTP-binding protein Di-Ras1 protein (1-195AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | IHC image of PACO60685 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
![]() | Immunofluorescence staining of A549 cells with PACO60685 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
Background: | Displays low GTPase activity and exists predominantly in the GTP-bound form. |
Synonyms: | GTP-binding protein Di-Ras1 (Distinct subgroup of the Ras family member 1) (Ras-related inhibitor of cell growth) (Rig) (Small GTP-binding tumor suppressor 1), DIRAS1, GBTS1 RIG |
UniProt Protein Function: | DIRAS1: Displays low GTPase activity and exist predominantly in the GTP-bound form. Belongs to the small GTPase superfamily. Di-Ras family.Protein type: G protein, monomeric, di-Ras; G protein; G protein, monomericChromosomal Location of Human Ortholog: 19p13.3Cellular Component: plasma membraneMolecular Function: GTP binding; GTPase activity; mitogen-activated protein kinase bindingBiological Process: positive regulation of MAP kinase activity |
UniProt Protein Details: | |
NCBI Summary: | DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004] |
UniProt Code: | O95057 |
NCBI GenInfo Identifier: | 62286591 |
NCBI Gene ID: | 148252 |
NCBI Accession: | O95057.1 |
UniProt Secondary Accession: | O95057 |
UniProt Related Accession: | O95057 |
Molecular Weight: | 22,329 Da |
NCBI Full Name: | GTP-binding protein Di-Ras1 |
NCBI Synonym Full Names: | DIRAS family GTPase 1 |
NCBI Official Symbol: | DIRAS1 |
NCBI Official Synonym Symbols: | RIG; GBTS1; Di-Ras1 |
NCBI Protein Information: | GTP-binding protein Di-Ras1 |
UniProt Protein Name: | GTP-binding protein Di-Ras1 |
UniProt Synonym Protein Names: | Distinct subgroup of the Ras family member 1; Ras-related inhibitor of cell growth; Rig; Small GTP-binding tumor suppressor 1 |
Protein Family: | GTP-binding protein |
UniProt Gene Name: | DIRAS1 |
UniProt Entry Name: | DIRA1_HUMAN |