Name: DIO2 Antibody (PACO19574)
SKU: PACO19574
Availability: OutOfStock

Antibody Name:	DIO2 Antibody (PACO19574)
Antibody SKU:	PACO19574
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB
Recommended Dilutions:	ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Synthetic peptide of human DIO2

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Gel: 6%SDS-PAGE, Lysate: 50 μg, Lane: Mouse brain tissue, Primary antibody: PACO19574(DIO2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds.

Background:

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It is highly expressed in the thyroid, and may contribute significantly to the relative increase in thyroidal T3 production in patients with Graves disease and thyroid adenomas. This protein contains selenocysteine (Sec) residues encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in multiple transcript variants encoding different isoforms.

Synonyms:

deiodinase, iodothyronine, type II

UniProt Protein Function:	DIO2: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Belongs to the iodothyronine deiodinase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 1.97.1.10; Oxidoreductase; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q24.2-q24.3 Cellular Component: membrane; plasma membrane Molecular Function:selenium binding; thyroxine 5'-deiodinase activity; ubiquitin protein ligase binding Biological Process: thyroid hormone generation; thyroid hormone metabolic process
NCBI Summary:	The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is widely expressed, including in thyroid, placenta, pituitary and brain. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. It has also been reported to be highly expressed in thyroids of patients with Graves disease, and in follicular adenomas. The intrathyroidal T4 to T3 conversion by this enzyme may contribute significantly to the relative increase in thyroidal T3 production in these patients. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2016]
UniProt Code:	Q92813
NCBI GenInfo Identifier:	172045839
NCBI Gene ID:	1734
NCBI Accession:	Q92813.4
UniProt Secondary Accession:	Q92813,Q6B0A3, Q9HCP8, Q9P1W4, Q9UDZ1, B9EGK0, G3V315
UniProt Related Accession:	Q92813
Molecular Weight:	34,704 Da
NCBI Full Name:	Type II iodothyronine deiodinase
NCBI Synonym Full Names:	deiodinase, iodothyronine, type II
NCBI Official Symbol:	DIO2
NCBI Official Synonym Symbols:	D2; 5DII; SelY; DIOII; TXDI2
NCBI Protein Information:	type II iodothyronine deiodinase
UniProt Protein Name:	Type II iodothyronine deiodinase
UniProt Synonym Protein Names:	5DII; DIOII; Type 2 DI; Type-II 5'-deiodinase
Protein Family:	Type II iodothyronine deiodinase
UniProt Gene Name:	DIO2
UniProt Entry Name:	IOD2_HUMAN