DiMethyl-Histone H3-K4 Monoclonal Antibody
- SKU:
- CAB22143
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
- Applications:
- IF
- Applications:
- IP
Description
| Product Name: | DiMethyl-Histone H3-K4 Monoclonal Antibody |
| Product Code: | CAB22143 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Applications: | Western blotting, Immunofluorescence, Immunoprecipitation |
| Host Species: | Rabbit |
| Purification Method: | Affinity purification |
| Isotype: | IgG |
| Clone No: | ARC55489 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Tested Applications: | WB, IF/ICC, IP, ChIP, ChIP-seq, ELISA, DB, CUT&Tag |
| Key Applications: | Western blotting, Immunofluorescence, Immunoprecipitation |
| Recommended Dilution: | DB 1:2000-1:6000, WB 1:1000-1:5000, IF/ICC 1:50-1:200, IP 1:2000-1:6000, ChIP 1:50-1:200, ChIP-seq 1:50-1:200, CUT&Tag 10, ⁵ cells /1 μg |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Positive Samples: | HeLa, NIH/3T3, C6 |
| Cellular Location: | Chromosome, nucleus |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
| Immunogen: | A synthetic dimethylated peptide around K4 of human Histone H3 (NP_003520.1). |
| Sequence: | MARTK QTARK STGGK APRKQ LATKA ARKSA PATGG VKKPH RYRPG TVALR EIRRY QKSTE LLIRK LPFQR LVREI AQDFK TDLRF QSSAV MALQE ACEAY |
| Synonyms: | H3t, H3.4, H3/g, H3FT, H3C16, HIST3H3 |
| Calculated MW: | 16kDa |
| Observed MW: | 17kDa |
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