Description
Antibody Name: | DHX35 Antibody (PACO42174) |
Antibody SKU: | PACO42174 |
Size: | 50ug |
Host Species: | Rabbit |
Tested Applications: | ELISA, WB, IHC |
Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:20-1:200 |
Species Reactivity: | Human, Mouse |
Immunogen: | Recombinant Human Probable ATP-dependent RNA helicase DHX35 protein (1-300AA) |
Form: | Liquid |
Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Purification Method: | >95%, Protein G purified |
Clonality: | Polyclonal |
Isotype: | IgG |
Conjugate: | Non-conjugated |
![]() | Western blot. All lanes: DHX35 antibody at 12µg/ml. Lane 1: Mouse kidney tissue. Lane 2: Mouse liver tissue. Lane 3: K562 whole cell lysate. Lane 4: Hela whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 79, 76 kDa. Observed band size: 79 kDa. |
![]() | Immunohistochemistry of paraffin-embedded human testis tissue using PACO42174 at dilution of 1:100. |
![]() | Immunohistochemistry of paraffin-embedded human brain tissue using PACO42174 at dilution of 1:100. |
Background: | May be involved in pre-mRNA splicing. |
Synonyms: | Probable ATP-dependent RNA helicase DHX35 (EC 3.6.4.13) (DEAH box protein 35), DHX35, C20orf15 DDX35 |
UniProt Protein Function: | DHX35: May be involved in pre-mRNA splicing. Belongs to the DEAD box helicase family. DEAH subfamily.Protein type: RNA splicing; Spliceosome; Helicase; EC 3.6.4.13Chromosomal Location of Human Ortholog: 20q11.22-q12Molecular Function: ATP binding; ATP-dependent RNA helicase activityBiological Process: nuclear mRNA splicing, via spliceosome; RNA processing |
UniProt Protein Details: | |
NCBI Summary: | DEAD box proteins characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The function of this gene product which is a member of this family, has not been determined. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2010] |
UniProt Code: | Q9H5Z1 |
NCBI GenInfo Identifier: | 299829255 |
NCBI Gene ID: | 60625 |
NCBI Accession: | NP_001177738.1 |
UniProt Secondary Accession: | Q9H5Z1,Q5THR0, Q9H4H7, Q9H6T6, A2RTX3, B4E0J0, F5GXM6 |
UniProt Related Accession: | Q9H5Z1 |
Molecular Weight: | |
NCBI Full Name: | probable ATP-dependent RNA helicase DHX35 isoform 2 |
NCBI Synonym Full Names: | DEAH-box helicase 35 |
NCBI Official Symbol: | DHX35 |
NCBI Official Synonym Symbols: | DDX35; C20orf15; KAIA0875 |
NCBI Protein Information: | probable ATP-dependent RNA helicase DHX35 |
UniProt Protein Name: | Probable ATP-dependent RNA helicase DHX35 |
UniProt Synonym Protein Names: | DEAH box protein 35 |
Protein Family: | Probable ATP-dependent RNA helicase |
UniProt Gene Name: | DHX35 |
UniProt Entry Name: | DHX35_HUMAN |