DEPP1 Antibody (PACO60336)
- SKU:
- PACO60336
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | DEPP1 Antibody (PACO60336) |
| Antibody SKU: | PACO60336 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Protein DEPP1 protein (57-174AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO60336 diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | Immunofluorescence staining of HepG2 cells with PACO60336 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | May play a role in autophagy. |
| Synonyms: | Protein DEPP1 (Decidual protein induced by progesterone) (Fasting-induced gene protein) (FIG), DEPP1, C10orf10 DEPP FIG |
| UniProt Protein Function: | Acts as a critical modulator of FOXO3-induced autophagy via increased cellular ROS. |
| UniProt Protein Details: | |
| NCBI Summary: | The expression of this gene is induced by fasting as well as by progesterone. The protein encoded by this gene contains a t-synaptosome-associated protein receptor (SNARE) coiled-coil homology domain and a peroxisomal targeting signal. Production of the encoded protein leads to phosphorylation and activation of the transcription factor ELK1. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NTK1 |
| NCBI GenInfo Identifier: | 5901938 |
| NCBI Gene ID: | 11067 |
| NCBI Accession: | NP_008952.1 |
| UniProt Secondary Accession: | Q9NTK1,O94997, Q5T735, Q76MX8, B2R6A1 |
| UniProt Related Accession: | Q9NTK1 |
| Molecular Weight: | 23,406 Da |
| NCBI Full Name: | protein DEPP1 |
| NCBI Synonym Full Names: | DEPP1, autophagy regulator |
| NCBI Official Symbol: | DEPP1 |
| NCBI Official Synonym Symbols: | FIG; DEPP; Fseg; C10orf10 |
| NCBI Protein Information: | protein DEPP1 |
| UniProt Protein Name: | Protein DEPP1 |
| UniProt Synonym Protein Names: | Decidual protein induced by progesterone |
| Protein Family: | Protein |
| UniProt Gene Name: | DEPP1 |
| UniProt Entry Name: |
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