def Antibody (PACO28862)
- SKU:
- PACO28862
- Product Type:
- Antibody
- Reactivity:
- Bacteria - Escherichia coli
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | def Antibody (PACO28862) |
| Antibody SKU: | PACO28862 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:1000-1:5000 |
| Species Reactivity: | Escherichia coli |
| Immunogen: | Recombinant Escherichia coli Peptide deformylase protein (2-169AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot All lanes: def antibody at 2µg/ml + DH5alpha whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 20 kDa Observed band size: 20 kDa . |
| Background: | Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. |
| Synonyms: | Peptide deformylase (PDF) (EC 3.5.1.88) (Polypeptide deformylase), def, fms |
| UniProt Protein Function: | Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. |
| UniProt Protein Details: | |
| NCBI Summary: | The def gene is essential unless the fmt formylase gene is also inactivated. [More information is available at EcoGene: EG11440]. Peptide deformylase releases the formyl group from the amino terminal methionine residue of most nascent proteins . [More information is available at EcoCyc: EG11440]. |
| UniProt Code: | P0A6K3 |
| NCBI GenInfo Identifier: | 16131166 |
| NCBI Gene ID: | 947780 |
| NCBI Accession: | NP_417745.1 |
| UniProt Secondary Accession: | P0A6K3,P27251, Q2M6V1 |
| UniProt Related Accession: | P0A6K3,CAA54826 |
| Molecular Weight: | 21.2 kDa |
| NCBI Full Name: | peptide deformylase |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | def |
| NCBI Official Synonym Symbols: | ECK3273; fms; JW3248 |
| NCBI Protein Information: | peptide deformylase |
| UniProt Protein Name: | Peptide deformylase |
| UniProt Synonym Protein Names: | Polypeptide deformylase |
| Protein Family: | Plectasin |
| UniProt Gene Name: | def |
| UniProt Entry Name: | DEF_ECOLI |
