Antibody Name:	DDX42 Antibody (PACO37422)
Antibody SKU:	PACO37422
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:500
Species Reactivity:	Human
Immunogen:	Recombinant Human ATP-dependent RNA helicase DDX42 protein (519-819AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Immunohistochemistry of paraffin-embedded human tonsil tissue using PACO37422 at dilution of 1:100.
	Immunofluorescence staining of Hela cells with PACO37422 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
	Immunohistochemistry of paraffin-embedded human lung tissue using PACO37422 at dilution of 1:100.

Background:

ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.

Synonyms:

ATP-dependent RNA helicase DDX42 (EC 3.6.4.13) (DEAD box protein 42) (RNA helicase-like protein) (RHELP) (RNA helicase-related protein) (RNAHP) (SF3b DEAD box protein) (Splicing factor 3B-associated 125 kDa protein) (SF3b125), DDX42

UniProt Protein Function:	DDX42: ATP-dependent RNA helicase. Binds to partially double- stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm. Belongs to the DEAD box helicase family. DDX42 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Helicase; EC 3.6.4.13 Chromosomal Location of Human Ortholog: 17q23.3 Cellular Component: cytoplasm; membrane; nucleoplasm; nucleus Molecular Function:ATP-dependent RNA helicase activity; protein binding Biological Process: protein localization; RNA secondary structure unwinding
NCBI Summary:	This gene encodes a member of the Asp-Glu-Ala-Asp (DEAD) box protein family. Members of this protein family are putative RNA helicases, and are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q86XP3
NCBI GenInfo Identifier:	74750541
NCBI Gene ID:	11325
NCBI Accession:	Q86XP3.1
UniProt Secondary Accession:	Q86XP3,O75619, Q68G51, Q96BK1, Q96HR7, Q9Y3V8, A6NML1 A8KA43,
UniProt Related Accession:	Q86XP3
Molecular Weight:	90,068 Da
NCBI Full Name:	ATP-dependent RNA helicase DDX42
NCBI Synonym Full Names:	DEAD-box helicase 42
NCBI Official Symbol:	DDX42
NCBI Official Synonym Symbols:	RHELP; RNAHP; SF3B8; DDX42P; SF3b125
NCBI Protein Information:	ATP-dependent RNA helicase DDX42
UniProt Protein Name:	ATP-dependent RNA helicase DDX42
UniProt Synonym Protein Names:	DEAD box protein 42; RNA helicase-like protein; RHELP; RNA helicase-related protein; RNAHP; SF3b DEAD box protein; Splicing factor 3B-associated 125 kDa protein; SF3b125
Protein Family:	ATP-dependent RNA helicase
UniProt Gene Name:	DDX42
UniProt Entry Name:	DDX42_HUMAN