DDX18 Antibody (PACO61213)
- SKU:
- PACO61213
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | DDX18 Antibody (PACO61213) |
| Antibody SKU: | PACO61213 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IF:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human ATP-dependent RNA helicase DDX18 protein (32-110AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Immunofluorescence staining of Hela cells with PACO61213 at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Probable RNA-dependent helicase. |
| Synonyms: | ATP-dependent RNA helicase DDX18 (EC 3.6.4.13) (DEAD box protein 18) (Myc-regulated DEAD box protein) (MrDb), DDX18, cPERP-D |
| UniProt Protein Function: | DDX18: Probable RNA-dependent helicase. Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily.Protein type: EC 3.6.4.13; Nucleolus; RNA-binding; HelicaseChromosomal Location of Human Ortholog: 2q14.1Cellular Component: membrane; nucleolusMolecular Function: protein binding; ATP binding; ATP-dependent RNA helicase activityBiological Process: metabolic process |
| UniProt Protein Details: | |
| NCBI Summary: | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it is activated by Myc protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NVP1 |
| NCBI GenInfo Identifier: | 38327634 |
| NCBI Gene ID: | 8886 |
| NCBI Accession: | NP_006764.3 |
| UniProt Secondary Accession: | Q9NVP1,Q6GTZ9, Q6IAU4, Q92732, Q9BQB7 |
| UniProt Related Accession: | Q9NVP1 |
| Molecular Weight: | 75,407 Da |
| NCBI Full Name: | ATP-dependent RNA helicase DDX18 |
| NCBI Synonym Full Names: | DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 |
| NCBI Official Symbol: | DDX18 |
| NCBI Official Synonym Symbols: | MrDb |
| NCBI Protein Information: | ATP-dependent RNA helicase DDX18; DEAD box protein 18; Myc-regulated DEAD box protein; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 18 (Myc-regulated) |
| UniProt Protein Name: | ATP-dependent RNA helicase DDX18 |
| UniProt Synonym Protein Names: | DEAD box protein 18; Myc-regulated DEAD box protein; MrDb |
| Protein Family: | ATP-dependent RNA helicase |
| UniProt Gene Name: | DDX18 |
| UniProt Entry Name: | DDX18_HUMAN |
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