D-Lactic Acid/Lactate Assay Kit - Colorimetric (MAES0015)

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SKU:
MAES0015
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Animal Tissue
Sensitivity:
0.06 mmol/L
Range:
0.06-8.0 mmol/L
Assay Time:
20 min
Shipping:
Gel Pack
Research Area:
Glycolysis & Carbohydrates
Research Area:
Diabetes & Obesity
Research Area:
Food Safety & Analysis
Research Area:
Clinical Chemistry

Description

system_update_altDatasheet
Product Name:D-Lactic Acid/Lactate Assay Kit - Colorimetric
Product Code:MAES0015
Product Size:96 Assays
Sample Type:Serum, plasma, animal tissue
Assay Time:20 min
Instrument:Microplate reader
Sensitivity:0.06 mmol/L
Detection Range:0.06-8.0 mmol/L
Recovery Rate:99
Inter CV:7.7
Inter CV:3.8

D-Lactic acid is an isomer of L-lactic acid. D-lactic acid is a product of bacterial metabolism. Due to the metabolism of pyruvaldehyde, D-lactic acid is usually present in the blood of mammals. The increase in D-lactic acid concentration may be due to the production by excessive gastrointestinal microbial. Subclinical elevation of D-lactic acid is an indicator of sepsis and trauma. The accumulation of D-lactic acid can lead to D-lactic acidosis, which is manifested as unlocalized nervous system symptoms and encephalopathy symptoms.

Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D- lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.

Shelf Life:6 Months
Storage:2-8°C
Shipping:Gel Pack
Research Area:Glycolysis & Carbohydrates
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