Metabolism Assays

D-Lactate Assay Kit (Colorimetric) (BA0097)

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SKU:
BA0097
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Cell Culture Media
Research Area:
TCA Cycle
Research Area:
Diabetes & Obesity
Research Area:
Food Safety & Analysis
Research Area:
Plant & Environmental Stress
Research Area:
Clinical Chemistry

Description

ELISA Kit Technical ManualMSDS

D-Lactate Assay Kit - Information

Assay Genie's Colorimetric D-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Applications

For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.

D-Lactate Assay Kit - Key Features

  • Sensitive and accurate. Detection limit of 0.05 mM and linearity up to 2 mM D-lactate in 96-well plate assay. For cell culture samples containing phenol red: detection limit of 0.1 mM and linearity up to 1 mM D-lactate in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and at 20 min. Room temperature assay. No 37°C heater is needed.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

D-Lactate Assay Kit - Data Sheet

Kit IncludesAssay Buffer: 10 mL NAD Solution: 1 mL Enzyme A: 120 mL MTT Solution: 1.5 mL Enzyme B: 120 mL Standard: 1.0 mL 20 mM D-lactate
Kit RequiresPipeting (multi-channel) devices. Clear-bottom 96-well plates and plate reader.
Method of DetectionOD565 nm
Detection Limit0.05 mM
SamplesSerum, plasma, cell culture media etc
SpeciesAll
Protocol Length20 min
Size100 tests
StorageStore all reagents at -20°C
Shelf Life6 months

More Details

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.
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