Name: CUX1 Antibody (PACO58120)
SKU: PACO58120
Availability: OutOfStock

Antibody Name:	CUX1 Antibody (PACO58120)
Antibody SKU:	PACO58120
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500
Species Reactivity:	Human, Mouse, Rat
Immunogen:	Recombinant Human Protein CASP protein (50-210AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, Rat brain tissue, Rat liver tissue, Rat kidney tissue, Mouse brain tissue. All lanes: CUX1 antibody at 3.7µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 78, 73, 165, 162, 166, 154, 158, 148, 76 kDa. Observed band size: 78 kDa.

IHC image of PACO58120 diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:	May be involved in intra-Golgi retrograde transport.
Synonyms:	Protein CASP, CUX1, CUTL1

UniProt Protein Function:	CUTL1 iso1: Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Belongs to the CUT homeobox family. 8 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; Membrane protein, integral; DNA-binding Chromosomal Location of Human Ortholog: 7q22.1 Cellular Component: cytosol; Golgi apparatus; nucleoplasm; nucleus Molecular Function:protein-tyrosine kinase activity; sequence-specific DNA binding Biological Process: multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of dendrite morphogenesis; regulation of transcription from RNA polymerase II promoter
NCBI Summary:	The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]
UniProt Code:	Q13948
NCBI GenInfo Identifier:	85681028
NCBI Gene ID:	1523
NCBI Accession:	Q13948.2
UniProt Secondary Accession:	Q13948,Q53GU9, Q8TBS3, B3KWH3, B3KWH8, B4DZZ2, G3V1Z6 J3KPQ6,
UniProt Related Accession:	Q13948,P39880
Molecular Weight:	75,644 Da
NCBI Full Name:	Protein CASP
NCBI Synonym Full Names:	cut like homeobox 1
NCBI Official Symbol:	CUX1
NCBI Official Synonym Symbols:	CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
NCBI Protein Information:	protein CASP
UniProt Protein Name:	Protein CASP
Protein Family:	Homeobox protein
UniProt Gene Name:	CUX1
UniProt Entry Name:	CASP_HUMAN