Antibody Name:	CSNK2A3 Antibody (PACO61999)
Antibody SKU:	PACO61999
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC, IP
Recommended Dilutions:	ELISA:1:2000-1:10000, WB:1:1000-1:5000, IHC:1:200-1:500, IP:1:200-1:2000
Species Reactivity:	Human
Immunogen:	Recombinant Human Casein kinase II subunit α 3 protein (151-391AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Western Blot. Positive WB detected in: PC3 whole cell lysate, SH-SY5Y whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, Hela whole cell lysate, THP-1 whole cell lysate. All lanes: CSNK2A3 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 kDa. Observed band size: 46 kDa.
	Immunoprecipitating CSNK2A3 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PACO61999 in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000). Lane 2: PACO61999 (5µg) + Hela whole cell lysate (0.5mg). Lane 3: Hela whole cell lysate (20µg).
	IHC image of PACO61999 diluted at 1:400 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Background:

Probable catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acid, c residues C-terminal to the phosphorylated serine or threonine. Amplification-dependent oncogene; promotes cell proliferation and tumorigenesis by down-regulating expression of the tumor suppressor protein, PML. May play a role in the pathogenesis of the lung cancer development and progression.

Synonyms:

Casein kinase II subunit α 3 (CK II α 3) (EC 2.7.11.1) (Casein kinase II α 1 polypeptide pseudogene), CSNK2A3, CSNK2A1P

UniProt Protein Function:	CSNK2A3: Probable catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Amplification-dependent oncogene; promotes cell proliferation and tumorigenesis by down- regulating expression of the tumor suppressor protein, PML. May play a role in the pathogenesis of the lung cancer development and progression. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. Heterotetramer composed of two catalytic subunits (alpha chain and/or alpha' chain) and two regulatory subunits (beta chains). Interacts with PMLProtein type: Oncoprotein; EC 2.7.11.1Chromosomal Location of Human Ortholog: 11p15.3
UniProt Protein Details:
NCBI Summary:	This gene encodes a protein that is highly similar to the casein kinase II alpha protein. Casein kinase II is a serine/threonine protein kinase complex that phosphorylates numerous substrates including casein. The alpha subunit is the catalytic component of the complex. Mutations in this gene may be associated with a susceptibility to lung cancer. There are contradictory views among published reports of this gene as to whether or not it is a protein-coding gene or a processed pseudogene (PMIDs: 20625391, 20625391 and 10094393). [provided by RefSeq, Feb 2012]
UniProt Code:	Q8NEV1
NCBI GenInfo Identifier:	487524482
NCBI Gene ID:	283106
NCBI Accession:	Q8NEV1.2
UniProt Secondary Accession:	Q8NEV1
UniProt Related Accession:	Q8NEV1
Molecular Weight:	45,220 Da
NCBI Full Name:	Casein kinase II subunit alpha 3
NCBI Synonym Full Names:	casein kinase 2 alpha 3
NCBI Official Symbol:	CSNK2A3
NCBI Official Synonym Symbols:	CSNK2A1P
NCBI Protein Information:	casein kinase 2, alpha 1 polypeptide-like
UniProt Protein Name:	Casein kinase II subunit alpha 3
UniProt Synonym Protein Names:	Casein kinase II alpha 1 polypeptide pseudogene
Protein Family:	Casein kinase
UniProt Gene Name:	CSNK2A3
UniProt Entry Name:	CSK23_HUMAN