crp Antibody (PACO34794)

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SKU:
PACO34794
Product Type:
Antibody
Reactivity:
Bacteria - Escherichia coli
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated

Description

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Antibody Name:crp Antibody (PACO34794)
Antibody SKU:PACO34794
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA
Recommended Dilutions:
Species Reactivity:Escherichia coli
Immunogen:Recombinant Escherichia coli cAMP-activated global transcriptional regulator CRP protein (1-210AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Background:A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription of about 300 genes in about 200 operons and indirectly regulate the expression of about half the genome. There are 3 classes of CRP promoters; class I promoters have a single CRP-binding site upstream of the RNA polymerase (RNAP)-binding site, whereas in class II promoters the single CRP- and RNAP-binding site overlap, CRP making multiple contacts with RNAP. Class III promoters require multiple activator molecules, including at least one CRP dimer. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter elements into contact with RNAP. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. High levels of active CRP are detrimental to growth.Plays a major role in carbon catabolite repression (CCR). CCR involves cAMP, adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr). In the presence of glucose EIIA-Glc is dephosphorylated, and does not activate adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity. Also plays a role in many other processes
Synonyms:cAMP-activated global transcriptional regulator CRP (Catabolite activator protein) (CAP) (Catabolite gene activator) (cAMP receptor protein) (CRP) (cAMP regulatory protein), crp, cap csm
UniProt Protein Function:A global transcription regulator. Complexes with cyclic AMP (cAMP) which allosterically activates DNA binding (to consensus sequence 5'-AAATGTGATCTAGATCACATTT-3') to directly regulate the transcription of about 300 genes in about 200 operons and indirectly regulate the expression of about half the genome. There are 3 classes of CRP promoters; class I promoters have a single CRP-binding site upstream of the RNA polymerase (RNAP)-binding site, whereas in class II promoters the single CRP- and RNAP-binding site overlap, CRP making multiple contacts with RNAP. Class III promoters require multiple activator molecules, including at least one CRP dimer. It can act as an activator, repressor, coactivator or corepressor. Induces a severe bend in DNA (about 87 degrees), bringing upstream promoter elements into contact with RNAP. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP. High levels of active CRP are detrimental to growth (PubMed:16260780). Plays a major role in carbon catabolite repression (CCR). CCR involves cAMP, adenylate cyclase (cyaA), CRP and the EIIA-Glc component of the PTS (crr). In the presence of glucose EIIA-Glc is dephosphorylated, and does not activate adenylate cyclase, leading to reduced cAMP and thus decreased CRP activity. Also plays a role in many other processes (see PubMed:22573269).
UniProt Protein Details:
NCBI Summary:
UniProt Code:P0ACJ8
NCBI GenInfo Identifier:82583733
NCBI Gene ID:29925187
NCBI Accession:P0ACJ8.1
UniProt Secondary Accession:P0ACJ8,P03020, Q2M723
UniProt Related Accession:P0ACJ8
Molecular Weight:25
NCBI Full Name:cAMP-activated global transcriptional regulator CRP
NCBI Synonym Full Names:
NCBI Official Symbol:N172_RS07815  
NCBI Official Synonym Symbols:
NCBI Protein Information:cAMP-activated global transcriptional regulator CRP
UniProt Protein Name:cAMP-activated global transcriptional regulator CRP
UniProt Synonym Protein Names:Catabolite activator protein; CAP; Catabolite gene activator; cAMP receptor protein; CRP; cAMP regulatory protein
Protein Family:Cryparin
UniProt Gene Name:crp  
UniProt Entry Name:
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