Creatinine Assay Kit (BA0034)

Creatinine Assay Kit - Information

Assay Genie's creatinine assay kit is designed to measure creatinine directly in biological samples without any pretreatment. The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine. The intensity of the color, measured at 510nm, is directly proportional to creatinine concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw sample.

Applications

For quantitative determination of creatinine and evaluation of drug effects on creatinine metabolism.

Creatinine Assay Kit - Key Features

• Sensitive and accurate. Use 30 samples. Detection limit 0.10 mg/dL (8 ) creatinine in 96-well plate assay.
• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 5 min. Can be automated as a high-throughput assay for thousands of samples per day.
• Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in 96-well plate or cuvet.
• Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples.

Creatinine Assay Kit - Data Sheet
Kit Includes	Reagent A: 50 mL Reagent B: 50 mL Creatinine Standard: 1 mL 50 mg/dL
Kit Requires	Pipeting devices and accessories (e.g. multi-channel pipettor). Clear bottom 96-well plates and plate reader for the plate procedure. Spectrophotometer and cuvets for measuring OD 510nm for the cuvette procedure.
Method of Detection	OD510nm
Detection Limit	0.1 mg/dL (8 )
Samples	Urine, serum, plasma and biological preparations
Species	All
Protocol Length	20 min
Size	500 tests
Storage	Store Reagents A and B at 2-8°C and Creatinine Standard at €“20°C.
Shelf Life	12 months

More Details

Creatinine is synthesized in the body at a fairly constant rate from creatine, which is produced during muscle contractions from creatine phosphate. In the blood, creatinine is removed by filtration through the glomeruli of the kidney and is secreted into urine. In healthy individuals, creatinine secretion is independent of diet and is fairly constant. The creatinine clearance test has become one of the most sensitive tests for measuring glomerular filtration rate. In kidney disease, creatinine levels in the blood are elevated, whereas the creatinine clearance rate and hence the urine levels are diminished. Creatinine test is most widely used to assess kidney function. Simple, direct and automation-ready procedures for measuring creatinine concentration in biological samples are becoming popular in Research and Drug Discovery.