Description
COVID-19 Human IgG PharmaGenie ELISA Kit (SBRS1718)
| Product SKU: | SBRS1718 |
| Size: | 96T |
| Gene Names: | IGG |
| Target Species: | Human |
| Compatible Sample Types: | Dried Blood, Plasma, Saliva, Serum |
| Design Principle: | Indirect |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. For extended storage, it is recommended to store at -80°C. |
- SARS-CoV-2 N-protein coated 96-well microplate
- Wash Buffer
- Positive Control
- Biotinylated Anti-Human IgG
- HRP-Streptavidin concentrate
- TMB One-Step Substrate
- Stop Solution
- Assay Diluent
- 5X Sample Diluent
- 1X PBS (DBS version)
- Tween-20 (DBS version)
- Protease Inhibitor Cocktail (DBS version)
Other materials and equipment required:
The Assay Genie COVID-19 Human IgG PharmaGenie ELISA Kit (SBRS1718) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 ul to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Distilled or deionized water
- Prepare all reagents, samples and standards as instructed.
- Add 100 ul positive control or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
- Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 ul TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
- Add 50 ul Stop Solution to each well. Read at 450 nm immediately.
