COVID-19 Human IgG PharmaGenie ELISA Kit (SBRS1718)

1. SARS-CoV-2 N-protein coated 96-well microplate
2. Wash Buffer
3. Positive Control
4. Biotinylated Anti-Human IgG
5. HRP-Streptavidin concentrate
6. TMB One-Step Substrate
7. Stop Solution
8. Assay Diluent
9. 5X Sample Diluent
10. 1X PBS (DBS version)
11. Tween-20 (DBS version)
12. Protease Inhibitor Cocktail (DBS version)

Other materials and equipment required:

The Assay Genie COVID-19 Human IgG PharmaGenie ELISA Kit (SBRS1718) will require other equipment and materials to carry out the assay. Please see list below for further details.

• Microplate reader capable of measuring absorbance at 450 nm
• Precision pipettes to deliver 2 ul to 1 ml volumes
• Adjustable 1-25 ml pipettes for reagent preparation
• 100 ml and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Distilled or deionized water

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 ul positive control or sample to each well. Incubate 1 hour at room temperature.
3. Add 100 µl prepared Biotinylated Anti-Human IgG Antibody into each well. Incubate 30 minutes at room temperature.
4. Add 100 µl prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
5. Add 100 ul TMB One-Step Substrate Reagent to each well. Incubate 15 minutes at room temperature.
6. Add 50 ul Stop Solution to each well. Read at 450 nm immediately.