Metabolism Assays

Coenzyme A (CoA) Assay Kit (Colorimetric/Fluorometric) (BA0093)

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SKU:
BA0093
Product Type:
Assay
Detection Method:
Colorimetric
Detection Method:
Fluorometric
Instrument:
Microplate Reader
Sample Type:
Biological
Research Area:
Ferroptosis
Research Area:
Diabetes & Obesity

Description

ELISA Kit Technical ManualMSDS

Coenzyme A Assay Kit - Information

Assay Genie's Coenzyme A Assay Kit method provides a simple, two-step and high-throughput assay for measuring coenzyme A. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the coenzyme A concentration in the sample.

Applications

For quantitative determination of coenzyme A and evaluation of drug effects on coenzyme A metabolism.

Coenzyme A Assay Kit - Key Features

  • Sensitive. Use 10 samples. Linear detection range: colorimetric assay 5 - 1000 , fluorimetric assay 3 - 100 CoA.
  • Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day.

Coenzyme A Assay Kit - Data Sheet

Method of DetectionOD570nm, or FL530/585nm
Detection Limit3
SamplesBiological
SpeciesAll
Protocol Length60 min
Size100 tests
Shelf Life6 months

More Details

Coenzyme Ais involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of coenzymes A most crucial roles is the carrying and transferring of acyl groups.
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