Name: CNOT3 Antibody (PACO55430)
SKU: PACO55430
Availability: OutOfStock

Antibody Name:	CNOT3 Antibody (PACO55430)
Antibody SKU:	PACO55430
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:20-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human CCR4-NOT transcription complex subunit 3 protein (257-395AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

IHC image of PACO55430 diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with PACO55430 at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity.

Synonyms:

CCR4-NOT transcription complex subunit 3 (CCR4-associated factor 3) (Leukocyte receptor cluster member 2), CNOT3, KIAA0691 LENG2 NOT3

UniProt Protein Function:	NOT3: The CCR4-NOT complex functions as general transcription regulation complex. Belongs to the CNOT2/3/5 family. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 19q13.4Cellular Component: nucleus; cytosol; CCR4-NOT complexMolecular Function: protein bindingBiological Process: regulation of translation; poly(A) tail shortening; transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA-mediated gene silencing; gene expression; trophectodermal cell differentiation; mRNA catabolic process, deadenylation-dependent decay
UniProt Protein Details:
NCBI Summary:
UniProt Code:	O75175
NCBI GenInfo Identifier:	46395626
NCBI Gene ID:	4849
NCBI Accession:	O75175.1
UniProt Secondary Accession:	O75175,Q9NZN7, Q9UF76
UniProt Related Accession:	O75175
Molecular Weight:	81,872 Da
NCBI Full Name:	CCR4-NOT transcription complex subunit 3
NCBI Synonym Full Names:	CCR4-NOT transcription complex, subunit 3
NCBI Official Symbol:	CNOT3
NCBI Official Synonym Symbols:	NOT3; LENG2; NOT3H
NCBI Protein Information:	CCR4-NOT transcription complex subunit 3; CCR4-associated factor 3; leukocyte receptor cluster member 2; NOT3 (negative regulator of transcription 3, yeast) homolog
UniProt Protein Name:	CCR4-NOT transcription complex subunit 3
UniProt Synonym Protein Names:	CCR4-associated factor 3; Leukocyte receptor cluster member 2
Protein Family:
UniProt Gene Name:	CNOT3
UniProt Entry Name:	CNOT3_HUMAN