CNGB1 Antibody (PACO59085)
- SKU:
- PACO59085
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- Applications:
- WB
- Applications:
- IHC
- Applications:
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
| Antibody Name: | CNGB1 Antibody (PACO59085) |
| Antibody SKU: | PACO59085 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Cyclic nucleotide-gated cation channel beta-1 protein (131-253AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, 293T whole cell lysate. All lanes: CNGB1 antibody at 4.6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 140, 71, 33 kDa. Observed band size: 140 kDa. |
 | Immunofluorescence staining of A549 cells with PACO59085 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
 | IHC image of PACO59085 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Subunit of cyclic nucleotide-gated (CNG) channels, nonselective cation channels, which play important roles in both visual and olfactory signal transduction. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP. |
| Synonyms: | Cyclic nucleotide-gated cation channel beta-1 (Cyclic nucleotide-gated cation channel 4) (CNG channel 4) (CNG-4) (CNG4) (Cyclic nucleotide-gated cation channel gamma) (Cyclic nucleotide-gated cation channel modulatory subunit) (Cyclic nucleotide-gated channel beta-1) (CNG channel beta-1) (Glutamic acid, rich protein) (GARP), CNGB1, CNCG2 CNCG3L CNCG4 RCNC2 |
| UniProt Protein Function: | Subunit of cyclic nucleotide-gated (CNG) channels, nonselective cation channels, which play important roles in both visual and olfactory signal transduction. When associated with CNGA1, it is involved in the regulation of ion flow into the rod photoreceptor outer segment (ROS), in response to light-induced alteration of the levels of intracellular cGMP. |
| UniProt Protein Details: | |
| NCBI Summary: | In humans, the rod photoreceptor cGMP-gated cation channel helps regulate ion flow into the rod photoreceptor outer segment in response to light-induced alteration of the levels of intracellular cGMP. This channel consists of two subunits, alpha and beta, with the protein encoded by this gene representing the beta subunit. Defects in this gene are a cause of cause of retinitis pigmentosa type 45. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013] |
| UniProt Code: | Q14028 |
| NCBI GenInfo Identifier: | 208431781 |
| NCBI Gene ID: | 1258 |
| NCBI Accession: | NP_001129111.1 |
| UniProt Secondary Accession: | Q14028,O43636, Q13059, Q14029, Q9UMG2, H3BN09 |
| UniProt Related Accession: | Q14028 |
| Molecular Weight: | 139,179 Da |
| NCBI Full Name: | cyclic nucleotide-gated cation channel beta-1 isoform b |
| NCBI Synonym Full Names: | cyclic nucleotide gated channel beta 1 |
| NCBI Official Symbol: | CNGB1 |
| NCBI Official Synonym Symbols: | CNG4; GAR1; GARP; RP45; CNCG2; CNCG4; GARP2; RCNC2; RCNCb; CNCG3L; CNGB1B; RCNCbeta |
| NCBI Protein Information: | cyclic nucleotide-gated cation channel beta-1 |
| UniProt Protein Name: | Cyclic nucleotide-gated cation channel beta-1 |
| UniProt Synonym Protein Names: | Cyclic nucleotide-gated cation channel 4; CNG channel 4; CNG-4; CNG4; Cyclic nucleotide-gated cation channel gamma; Cyclic nucleotide-gated cation channel modulatory subunit; Cyclic nucleotide-gated channel beta-1; CNG channel beta-1; Glutamic acid-rich protein; GARP |
| Protein Family: | Cyclic nucleotide-gated cation channel |
| UniProt Gene Name: | CNGB1 |
| UniProt Entry Name: |
| Antibodies |
| CNGB1 Antibody (PACO06363) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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